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Guo Jiayi

A master student in Chemical Biology at University of Geneva and EPFL. Studying Machine Learning and Computional biology at this stage!

Structural Biology

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2. Menin “reads” H3K79me2 mark in a nucleosomal context

Xiang David Li,Zhai Yuanliang

Published in Science

Key words: Breakthrough on the Known phenomena, H3K73me2, Photocross-linking, Structral biology

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  • Comments: Identifying the “Reader” of H3K79me2 has puzzled the scientists for many years, but Xiang David Li and his collegues developed a Chemical probe method to identify this “Reader”, and the experiment design of this article is wonderful, they cleverly combined different analysis tools together, while they identified this “Reader”, they also described its working mechanism as detailed as possible.

4. Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold

Yeates, Todd O

Published in PNAS

Key words: Development on Existing technology, Structural Biology, small protein imaging, Protein assemblies

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  • Comments: Identifying the structure of small proteins with Crto-EM has confused scientists for a along time, this paper further improved the idea of making use of a bigger scaffold self-assembly protein to image the small protein, they rigidified the DARPin through modified the protrution direction of DARPin and making it less flexible. To validate its reliability, the author analyse the Cryo-EM structure of KRAS protein and get a good resolution. But the author do not mention any limitations of this technology, for example, the drug can not bind to where the DARPin binds to.